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Image via Complex Original 25. Captain Marvel (Marvel Super Heroes #12, 1967)
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Image via Complex Original 24. Mary Jane Watson (The Amazing Spider-Man #42, 1966 – First full appearance)
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Image via Complex Original 23. Hawkeye (Tales of Suspense #57, 1964)
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Image via Complex Original 22. Ant-Man and the Wasp (Ant-Man: Tales to Astonish #27, 1962; Wasp: Tales to Astonish #44, 1963)
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Image via Complex Original 21. She-Hulk (Savage She-Hulk #1, 1980)
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Image via Complex Original 20. Black Widow (Tales of Suspense #52, 1964)
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Image via Complex Original 19. Norman Osborn a.k.a. The Green Goblin (Amazing Spider-Man #14, 1964)
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Image via Complex Original 18. The Inhumans (Fantastic Four #45, 1965)
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Image via Complex Original 17. Nick Fury (Sgt. Fury and his Howling Commandos #1, 1963)
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Image via Complex Original 16. Odin (Journey into Mystery #86, 1962)
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Image via Complex Original 15. Magneto (The X-Men #1, 1963)
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Image via Complex Original 14. Loki (Journey into Mystery #85, 1962)
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Image via Complex Original 13. Galactus (Fantastic Four #48, 1966)
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Image via Complex Original 12. Black Panther (Fantastic Four #52, 1966)
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Image via Complex Original 11. Doctor Doom (Fantastic Four #5, 1962)
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Image via Complex Original 10. Doctor Strange (Strange Tales #110, 1963)
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Image via Complex Original 9. Daredevil (Daredevil #1, 1964)
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Image via Complex Original 8. The Silver Surfer (Fantastic Four #48, 1966)
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Image via Complex Original 7. Thor (Journey into Mystery #83, 1962)
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Image via Complex Original 6. X-Men (The X-Men #1, 1963)
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Image via Complex Original 5. Iron Man (Tales of Suspense #39, 1963)
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Image via Complex Original 4. The Hulk (The Incredible Hulk #1, 1962)
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Image via Complex Original 3. The Avengers (The Avengers #1, 1963)
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Image via Complex Original 2. Fantastic Four (Fantastic Four #1, 1961)
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Image via Complex Original 1. Spider-Man (Amazing Fantasy #15, 1962)
